ProteoSure™ Modified, Recombinant Porcine Trypsin, Autolysis-Resistant for Proteomic Sequencing and Mass Spectrometry Analysis

ProteoSure™ Modified, Recombinant Porcine Trypsin, Autolysis-Resistant for Proteomic Sequencing and Mass Spectrometry AnalysisProduct highlights Enables fast digestion and reduces wait time in sample preparation; Free of non tryptic activities for high quality, unambiguous mass spectral interpretation; Provides enhanced peptide map coverage; Suits a wide range of proteomics applications. Product overview Trypsin is a crucial serine protease that specifically cleaves peptide bonds at the carboxyl side of lysine and arginine residues. It is widely utilized in mass spectrometry

coli Binding capacity >15 mg human IgG/mL medium Matrix Cross-linked 4% agarose supplied as a 50% slurry Particle size 45 μm - 165 μm Maximum pressure

Storage Store in a sealed container at 2 - 8°C

13 mmol H+ /mL medium Matrix Highly cross-linked 6% agarose supplied as a 50% slurry Particle size 45 μm - 165 μm Flow rate

The pre-activated functional N-hydroxysuccinimide (NHS) group forms a chemically stable amide bone with the ligand that contains a primary amino group

we provide stopcocks made of different materials

It has a calculated molecular weight of 34 kDa

Size exclusion chromatography (SEC)

Manual batch or gravity flow purification

CL: raw lysate

Fractions were analyzed by SDS-PAGE

8 kDa Origin of species Human Source E

The ratio of Trypsin to the protein sample should be between 1:20 and 1:100 (w/w)