GraviPur™15 Desalting Column with G-25 Medium Size Exclusion Resin for cleanup of 15 mL sample

GraviPur™15 Desalting Column with G-25 Medium Size Exclusion Resin for cleanup of 15 mL sampleGraviPur Desalting Columns are prepacked with PROTEINDEX SEC G 25 Cross linked Dextran Resin, a molecular weightbased selection resin designed for rapid and efficient removal of small molecules. With an effective cutoff of approximately Mr 5,000, these columns enable biomolecules larger than the exclusion limitsuch as proteins, peptides, nucleic acids, and other macromoleculesto be cleanly separated from salts, detergents, and other lowmolecular

≥170 Units/mg protein Unit definition One unit of carboxypeptidase B activity hydrolyzes one micromole of hippuryl-L-arginine per minute at 25℃

Aliquots of the stock solution may be stored at -20°C for up to 6 months

Denaturing agents:

or a tubing connected to a male luer slip adapter

even after multiple cycles of CIP with 1 M NaOH

time-consuming

Coupling capacity > 10 mg IgG/mL medium Matrix Highly cross-linked 4% agarose supplied as a 50% slurry Particle size 45 μm - 165 μm

Product package size 5 columns Medium ProteIndex™ SEC G-25 Medium Cross-Linked Dextran Column bed volume

4 mm Column height 134

Temperature: 25°C

a step of removing incompatible agents to Ni-NTA resins by dialysis is frequently required prior to loading the material on the column

The resin is well suited for batch/gravity purification