CRISPR Non-Targeting sgRNA Control Construct in Tet-Inducible Vector (no Cas9)

CRISPR Non-Targeting sgRNA Control Construct in Tet-Inducible Vector (no Cas9)Tet inducible non targeting sgRNA control in a lentiviral vector (no Cas9). The tet inducible U6 promoter drives sgRNA expression and is responsive to the tetracycline analog doxocycline. Vector is available with either GFP or RFP fluorescence and puro selection. Catalog # Description Vector Type SGCTL NT pRSGT16 CMV_RFP_Puro Non Targeting CRISPR control in pRSGT16 U6Tet sg CMV TetR RFP Puro plasmid SGCTL NT pRSGT16 V CMV_RFP_Puro Non Targeting CRISPR

Non-Targeting CRISPR control in pRSG16-U6-sg-UbiC-RFP-Puro

or similar rotor requires an additional hour

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the RFP is co-expressed and can be easily detected

Applications for DriverMap AIR RNA Spike-in Controls

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or dCas9-activator by measuring loss of GFP in any mammalian cell line

DM-NGS-SetA-B2  Unique Dual Index Set A for DriverMap EXP or AIR (1-96)  1x 96-Well Plate (4 rxn/well)   Order Now > DM-NGS-SetB-B2  Unique Dual Index Set B for DriverMap EXP or AIR (97-192)  1x 96-Well Plate (4 rxn/well)   Order Now > DM-NGS-SetC-B2  Unique Dual Index Set C for DriverMap EXP or AIR (193-288)  1x 96-Well Plate (4 rxn/well)   Order Now > DM-NGS-SetD-B2  Unique Dual Index Set D for DriverMap EXP or AIR (289-384)  1x 96-Well Plate (4 rxn/well)   Order Now >

CRISPR screening of livestock and poultry could be useful in the study of pathogen resistance

Choose from a range of constructs with various selection markers

Flyer: Comprehensive Adaptive Immune Receptor Profiling for All Immune Sample Types

Choose 5 or 10 lentiviral constructs with barcodes flanked by common primer sequences to facilitate PCR amplifications